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MXRA8 ELISA Kit (Human)

CAT: 0247-OKCA01352-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA01352-96WSize:96 Well
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Matrix remodeling associated 8
Gene Aliases
ASP3
Gene ID
54587
Accession Number
NP_001269511.1
Reactivity
Human
Target
May play a role in the maturation and maintenance of blood-brain barrier.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology MXRA8 ELISA Kit (Human) (OKCA01352) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MXRA8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MXRA8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MXRA8 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.156 - 10 ng/mL
Recovery
Sample Mean Recovery (%) Range (%) Serum (n=5) 91 82-94 EDTA Plasma (n=4) 103 94-106 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 91 82-94 Serum (n=5) 91 82-94 EDTA Plasma (n=4) 103 94-106 EDTA Plasma (n=4) 103 94-106
Sensitivity
0.039 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
MXRA8
Protein Name
Matrix-remodeling-associated protein 8
Gene Name URL
MXRA8
Nucleotide Accession Number
NM_001282582.1

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