IGF2BP2 ELISA Kit (Human)

CAT: 0247-OKCA01313-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCA01313-96WSize:96 Well
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Insulin like growth factor 2 mRNA binding protein 2
Gene Aliases
IMP2, IMP-2, VICKZ2
Gene ID
10644
Accession Number
NP_001007226.1
Reactivity
Human
Target
RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs) . This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology IGF2BP2 ELISA Kit (Human) (OKCA01313) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGF2BP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IGF2BP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IGF2BP2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Lysates
Detection Range
125 - 8,000 pg/mL
Recovery
Sample Mean Recovery (%) Range (%) _x005F Serum (n=5) 89 84-93 _x005F EDTA Plasma (n=4) 95 90-100 _x005F Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 89 84-93 Serum (n=5) 89 84-93 EDTA Plasma (n=4) 95 90-100 EDTA Plasma (n=4) 95 90-100
Sensitivity
31.25 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
IGF2BP2
Protein Name
Insulin-like growth factor 2 mRNA-binding protein 2
Gene Name URL
IGF2BP2
Nucleotide Accession Number
NM_001007225.2

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