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AVP ELISA Kit (Sheep)

CAT: 0247-OKCA01026-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA01026-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Vasopressin-neurophysin 2-copeptin
Accession Number
NP_001119813.1
Reactivity
Sheep
Target
Neurophysin 2 specifically binds vasopressin. Vasopressin has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology AVP ELISA Kit (Sheep) (OKCA01026) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for AVP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AVP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample AVP captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
1.56 pg/mL-100 pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 95 89-98 EDTA Plasma (n=4) 92 86-97 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 95 89-98 Serum (n=5) 95 89-98 EDTA Plasma (n=4) 92 86-97 EDTA Plasma (n=4) 92 86-97
Sensitivity
0.39 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
AVP
Protein Name
Vasopressin-neurophysin 2-copeptin
Gene Name URL
AVP
Nucleotide Accession Number
NM_001126341.1

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