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B2M ELISA Kit (Rat)

CAT: 0247-OKCA01018-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA01018-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Beta-2-microglobulin
Accession Number
XP_008267300.1
Reactivity
Rat
Target
Component of the class I major histocompatibility complex (MHC) . Involved in the presentation of peptide antigens to the immune system.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology B2M ELISA Kit (Rat) (OKCA01018) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for B2M has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for B2M is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample B2M captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine And Tissue Homogenates
Detection Range
0.156 ug/mL-10 ug/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 97 90-104 EDTA Plasma (n=4) 93 89-97 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 97 90-104 Serum (n=5) 97 90-104 EDTA Plasma (n=4) 93 89-97 EDTA Plasma (n=4) 93 89-97
Sensitivity
0.039 ug/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
B2M
Protein Name
Beta-2-microglobulin
Gene Name URL
B2M
Nucleotide Accession Number
XM_008269078.2

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