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EPO ELISA Kit (Rat)

CAT: 0247-OKCA00971-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00971-96WSize:96 Well
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Erythropoietin
Gene Aliases
Erythropoietin
Gene ID
100008786
Accession Number
NP_001075559.1
Reactivity
Rat
Target
Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology EPO ELISA Kit (Rat) (OKCA00971) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for EPO has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EPO is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample EPO captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 96 92-100 EDTA Plasma (n=4) 95 86-99 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 96 92-100 Serum (n=5) 96 92-100 EDTA Plasma (n=4) 95 86-99 EDTA Plasma (n=4) 95 86-99
Sensitivity
0.039 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
EPO
Protein Name
Erythropoietin
Gene Name URL
EPO
Nucleotide Accession Number
NM_001082090.1

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