FSHB ELISA Kit (rabbit)

• Reproducibility:

  Inter-Assay CV:

• Gene Name:

  Follitropin subunit beta

• Gene Aliases:

  FSH

• Gene ID:

  100008946

• Accession Number:

  NP_001075640.1

• Reactivity:

  Rabbit

• Target:

  Stimulates development of follicle and spermatogenesis in the reproductive organs.

• Type:

  ELISA Kit

• Assay Principle:

  Aviva Systems Biology FSHB ELISA Kit (rabbit) (OKCA00938) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for FSHB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FSHB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample FSHB captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates

• Detection Range:

  1.56 mIU/mL-100 mIU/mL

• Recovery:

  Matrix Recovery range (%) Average (%) Serum (n=5) 94 89-98 EDTA Plasma (n=4) 96 90-105 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 94 89-98 Serum (n=5) 94 89-98 EDTA Plasma (n=4) 96 90-105 EDTA Plasma (n=4) 96 90-105

• Sensitivity:

  0.39 mIU/mL

• Reconstitution:

  Store as indicated in product manual.

• NCBI Gene Symbol:

  FSHB

• Protein Name:

  Follitropin subunit beta

• Gene Name URL:

  FSHB

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001082171.2