VSIG4 ELISA Kit (Human)

CAT: 0247-OKCA00860-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCA00860-96WSize:96 Well
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
V-set and immunoglobulin domain containing 4
Gene Aliases
CRIg, Z39IG
Gene ID
11326
Accession Number
NP_001093901.1
Reactivity
Human
Target
Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology VSIG4 ELISA Kit (Human) (OKCA00860) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for VSIG4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for VSIG4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample VSIG4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Lysates
Detection Range
31.25 pg/mL-2,000 pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 95 89-97 EDTA Plasma (n=4) 90 86-94 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 95 89-97 Serum (n=5) 95 89-97 EDTA Plasma (n=4) 90 86-94 EDTA Plasma (n=4) 90 86-94
Sensitivity
7.81 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
VSIG4
Protein Name
V-set and immunoglobulin domain-containing protein 4
Gene Name URL
VSIG4
Nucleotide Accession Number
NM_001100431.1

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