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LIPA ELISA Kit (Human)

CAT: 0247-OKCA00718-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00718-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Lipase A, lysosomal acid type
Gene Aliases
LAL, CESD
Gene ID
3988
Accession Number
NP_000226.2
Reactivity
Human
Target
Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology LIPA ELISA Kit (Human) (OKCA00718) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LIPA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LIPA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LIPA captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates And Cell Lysates
Detection Range
62.5 pg/mL-4,000 pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 86 82-90 EDTA Plasma (n=4) 94 90-100 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 86 82-90 Serum (n=5) 86 82-90 EDTA Plasma (n=4) 94 90-100 EDTA Plasma (n=4) 94 90-100
Sensitivity
15.6 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
LIPA
Protein Name
Lysosomal acid lipase/cholesteryl ester hydrolase
Gene Name URL
LIPA
Nucleotide Accession Number
NM_000235.3

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