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MX1 ELISA Kit (Human)

CAT: 0247-OKCA00704-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00704-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
MX dynamin like GTPase 1
Gene Aliases
MX, MxA, IFI78, IFI-78K, lncMX1-215
Gene ID
4599
Accession Number
NP_001138397.1
Reactivity
Human
Target
This gene encodes a guanosine triphosphate (GTP) -metabolizing protein that participates in the cellular antiviral response. The encoded protein is induced by type I and type II interferons and antagonizes the replication process of several different RNA and DNA viruses. There is a related gene located adjacent to this gene on chromosome 21, and there are multiple pseudogenes located in a cluster on chromosome 4. Alternative splicing results in multiple transcript variants.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology MX1 ELISA Kit (Human) (OKCA00704) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MX1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MX1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MX1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates, Cell Lysates
Detection Range
15.6 pg/mL-1,000 pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 95 90-99 EDTA Plasma (n=4) 98 90-102 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 95 90-99 Serum (n=5) 95 90-99 EDTA Plasma (n=4) 98 90-102 EDTA Plasma (n=4) 98 90-102
Sensitivity
3.9 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
MX1
Protein Name
Interferon-induced GTP-binding protein Mx1
Gene Name URL
MX1
Nucleotide Accession Number
NM_001144925.2

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