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GALT ELISA Kit (Human)

CAT: 0247-OKCA00681-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00681-96WSize:96 Well
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Galactose-1-phosphate uridylyltransferase
Gene Aliases
Gal-1-P uridylyltransferase, Galactose-1-phosphate uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase
Gene ID
2592
Accession Number
NP_000146.2
Reactivity
Human
Target
Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology GALT ELISA Kit (Human) (OKCA00681) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GALT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GALT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample GALT captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
31.25 pg/mL-2,000 pg/mL
Recovery
_x005F Matrix Recovery range (%) Average (%) _x005F Serum (n=5) 90 84-94 _x005F EDTA Plasma (n=4) 94 88-97 _x005F Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 90 84-94 Serum (n=5) 90 84-94 EDTA Plasma (n=4) 94 88-97 EDTA Plasma (n=4) 94 88-97
Sensitivity
7.81 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
GALT
Protein Name
Galactose-1-phosphate uridylyltransferase
Gene Name URL
GALT
Nucleotide Accession Number
NM_000155.3

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