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CPB2 ELISA Kit (Human)

CAT: 0247-OKCA00124-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00124-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay: CV% < 8% (n = 20) .Inter-assay: CV% < 10% (n = 20)
CAS Number
7732-18-5
Gene Name
Carboxypeptidase B2
Gene Aliases
CPU, PCPB, TAFI
Gene ID
1361
Accession Number
NP_001265470
Reactivity
Human
Target
Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues) . The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Type
ELISA Kit
Assay Principle
Aviva Systems Biology CPB2 ELISA Kit (Human) (OKCA00124) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for human CPB2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for human CPB2 is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample human CPB2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Plasma, Tissue Homogenates
Detection Range
31.25 ng/mL - 2,000 ng/mL
Recovery
Sample 1 Sample Type Average Recovery Range Serum (n=5) 98% 92-104% Sample 1 Sample 1 Sample Type Average Recovery Range Sample Type Average Recovery Range Serum (n=5) 98% 92-104% Serum (n=5) 98% 92-104% Sample 2 Sample Type Average Recovery Range EDTA Plasma (n=4) 92% 89-95% Sample 2 Sample 2 Sample Type Average Recovery Range Sample Type Average Recovery Range EDTA Plasma (n=4) 92% 89-95% EDTA Plasma (n=4) 92% 89-95%
Sensitivity
< 7.81 ng/ml
Reconstitution
Store as indicated in product manual.
Molecular Weight
48 kDa
Protein Length
423
NCBI Gene Symbol
CPB2
Protein Name
Carboxypeptidase B2
Gene Name URL
CPB2
Nucleotide Accession Number
NM_001278541

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