RBP4 ELISA Kit (Human)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 621 2874 9419 643 2778 10172 Standard deviation 44.09 227.04 668.74 53.36 247.24 752.72 CV (%) 7.1 7.9 7.1 8.3 8.9 7.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 621 2874 9419 643 2778 10172 Mean (pg/ml) 621 2874 9419 643 2778 10172 Standard deviation 44.09 227.04 668.74 53.36 247.24 752.72 Standard deviation 44.09 227.04 668.74 53.36 247.24 752.72 CV (%) 7.1 7.9 7.1 8.3 8.9 7.4 CV (%) 7.1 7.9 7.1 8.3 8.9 7.4

• Gene Name:

  Retinol-binding protein 4

• Gene Aliases:

  Retinol-binding protein 4; Plasma retinol-binding protein; PRBP; RBP; Plasma retinol-binding protein (1-182) ; Plasma retinol-binding protein (1-181) ; Plasma retinol-binding protein (1-179) ; Plasma retinol-binding protein (1-176) ; RBP4; PRO2222

• Gene ID:

  5950

• Accession Number:

  NP_001310446.1

• Reactivity:

  Human

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: E19-L201

• Target:

  Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It is protein that in humans is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c (Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's human RBP4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for RBP4 has been precoated onto 96-well plates. Standards (NS0, E19-L201) and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for RBP4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human RBP4 amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernates, serum, plasma (heparin , EDTA) and urine

• Detection Range:

  312 pg/ml - 20,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant human RBP4

• NCBI Gene Symbol:

  RBP4

• Protein Name:

  Retinol-binding protein 4

• Gene Name URL:

  RBP4

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001323517.1