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CSF1 ELISA Kit (Human)

CAT: 0247-OKBB01491-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01491-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 152 681 1139 140 716 1136 Standard deviation 8.05 32.68 52.39 9.1 40.09 52.25 CV (%) 5.3 4.8 4.6 6.5 5.6 4.6 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 152 681 1139 140 716 1136 Mean (pg/ml) 152 681 1139 140 716 1136 Standard deviation 8.05 32.68 52.39 9.1 40.09 52.25 Standard deviation 8.05 32.68 52.39 9.1 40.09 52.25 CV (%) 5.3 4.8 4.6 6.5 5.6 4.6 CV (%) 5.3 4.8 4.6 6.5 5.6 4.6
CAS Number
7732-18-5
Gene Name
Macrophage colony-stimulating factor 1
Gene Aliases
Macrophage colony-stimulating factor 1; CSF-1; M-CSF; MCSF; Lanimostim; Processed macrophage colony-stimulating factor 1; CSF1
Gene ID
1435
Accession Number
NP_000748.3
Reactivity
Human
Immunogen
Expression system for standard: E.coli; Immunogen sequence: E33-S190
Target
M-CSF, also called CSF1, has a role in development of the placenta. Uterine CSF1 concentration is regulated by a synergistic action of estradiol and progesterone. CSF1 is produced by uterine glandular epithelial cells. It had been found that FMS, the CSF1 receptor, is expressed in placenta and choriocarcinoma cell lines1. The CSF1 gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb2. And there are 2 forms of CSF1, with 224 and 522 amino acids, resulting from alternative splicing3.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human M-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for M-CSF has been precoated onto 96-well plates. Standards (E.coli, E33-S190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for M-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human M-CSF amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human M-CSF
NCBI Gene Symbol
CSF1
Protein Name
Macrophage colony-stimulating factor 1
Gene Name URL
CSF1
Nucleotide Accession Number
NM_000757.5

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