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ANGPTL2 ELISA Kit (Human)

CAT: 0247-OKBB01406-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01406-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 590 1670 7933 594 1958 8471 Standard deviation 30.68 71.81 325.25 39.20 113.56 559.09 CV (%) 5.2 4.3 4.1 6.6 5.8 6.6 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 590 1670 7933 594 1958 8471 Mean (pg/ml) 590 1670 7933 594 1958 8471 Standard deviation 30.68 71.81 325.25 39.20 113.56 559.09 Standard deviation 30.68 71.81 325.25 39.20 113.56 559.09 CV (%) 5.2 4.3 4.1 6.6 5.8 6.6 CV (%) 5.2 4.3 4.1 6.6 5.8 6.6
CAS Number
7732-18-5
Gene Name
Angiopoietin like 2
Gene Aliases
Angiopoietin-related protein 2; Angiopoietin-like protein 2
Gene ID
23452
Accession Number
NP_036230.1
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q23-H493
Target
Angiopoietin-related protein 2 also known as angiopoietin-like protein 2 is a protein that in humans is encoded by the ANGPTL2 gene. This gene is mapped to 9q33.3. Angiopoietin-like protein 2 maintains tissue homeostasis by promoting adaptive inflammation and subsequent tissue reconstruction, whereas an excess of ANGPTL2 activation induced by prolonged stress promotes the breakdown of tissue homeostasis due to chronic inflammation, promoting the development of metabolic diseases. ANGPTL2 has a role also in angiogenesis, in tissue repair, in obesity, in atherosclerotic diseases and finally in carcinogenesis. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human ANGPTL2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ANGPTL2 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: Q23-H493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANGPTL2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human ANGPTL2 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
312 pg/ml - 20,000 pg/ml
Sensitivity
<50 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human ANGPTL2
NCBI Gene Symbol
ANGPTL2
Protein Name
Angiopoietin-related protein 2
Gene Name URL
ANGPTL2
Nucleotide Accession Number
NM_012098.2

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