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Stc2 ELISA Kit (Mouse)

CAT: 0247-OKBB01359-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01359-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 1255 4504 284 1264 4728 Standard deviation 12.84 56.48 256.73 19.60 73.31 297.86 CV (%) 5.2 4.5 5.7 6.9 5.8 6.3 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 247 1255 4504 284 1264 4728 Mean (pg/ml) 247 1255 4504 284 1264 4728 Standard deviation 12.84 56.48 256.73 19.60 73.31 297.86 Standard deviation 12.84 56.48 256.73 19.60 73.31 297.86 CV (%) 5.2 4.5 5.7 6.9 5.8 6.3 CV (%) 5.2 4.5 5.7 6.9 5.8 6.3
CAS Number
7732-18-5
Gene Name
Stanniocalcin 2
Gene Aliases
Stanniocalcin-2; STC-2; Stc2
Gene ID
20856
Accession Number
NP_035621.1
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: T25-R296
Target
Stanniocalcin-2 is a protein that in humans is encoded by the STC2 gene. It is mapped to 11; 11 A4. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse STC2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for STC2 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: T25-R296) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for STC2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substmousee TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<50 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse STC2
NCBI Gene Symbol
STC2
Protein Name
Stanniocalcin-2
Gene Name URL
STC2
Nucleotide Accession Number
NM_011491.3

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