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CNDP1 ELISA Kit (Human)

CAT: 0247-OKBB01334-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01334-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 613 2702 8855 620 2918 9790 Standard deviation 30.04 143.21 602.14 44.02 131.31 528.66 CV (%) 4.9 5.3 6.8 7.1 4.5 5.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 613 2702 8855 620 2918 9790 Mean (pg/ml) 613 2702 8855 620 2918 9790 Standard deviation 30.04 143.21 602.14 44.02 131.31 528.66 Standard deviation 30.04 143.21 602.14 44.02 131.31 528.66 CV (%) 4.9 5.3 6.8 7.1 4.5 5.4 CV (%) 4.9 5.3 6.8 7.1 4.5 5.4
CAS Number
7732-18-5
Gene Name
Carnosine dipeptidase 1
Gene Aliases
Beta-Ala-His dipeptidase; CNDP dipeptidase 1; Carnosine dipeptidase 1; Glutamate carboxypeptidase-like protein 2; Serum carnosinase; CNDP1; CN1; CPGL2; UNQ1915/PRO4380
Gene ID
84735
Accession Number
NP_116038.4
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: S27-H507
Target
Beta-Ala-His dipeptidase is an enzyme that in humans is encoded by the CNDP1 gene. It is mapped to 18q22.3. This gene encodes a member of the M20 metalloprotease family. The encoded protein is specifically expressed in the brain, is a homodimeric dipeptidase which was identified as human carnosinase. This gene contains trinucleotide (CTG) repeat length polymorphism in the coding region.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human CNDP1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CNDP1 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: S27-H507) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CNDP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
312 pg/ml - 20,000 pg/ml
Sensitivity
<50 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human CNDP1
NCBI Gene Symbol
CNDP1
Protein Name
Beta-Ala-His dipeptidase
Gene Name URL
CNDP1
Nucleotide Accession Number
NM_032649.5

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