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CREG1 ELISA Kit (Human)

CAT: 0247-OKBB01333-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01333-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 441 1500 8633 514 2089 9813 Standard deviation 21.61 79.50 578.41 32.90 100.27 578.97 CV (%) 4.9 5.3 6.7 6.4 4.8 5.9 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 441 1500 8633 514 2089 9813 Mean (pg/ml) 441 1500 8633 514 2089 9813 Standard deviation 21.61 79.50 578.41 32.90 100.27 578.97 Standard deviation 21.61 79.50 578.41 32.90 100.27 578.97 CV (%) 4.9 5.3 6.7 6.4 4.8 5.9 CV (%) 4.9 5.3 6.7 6.4 4.8 5.9
CAS Number
7732-18-5
Gene Name
Cellular repressor of E1A stimulated genes 1
Gene Aliases
Protein CREG1; Cellular repressor of E1A-stimulated genes 1; CREG1; CREG; UNQ727/PRO1409
Gene ID
8804
Accession Number
NP_003842.1
Reactivity
Human
Immunogen
Expression system for standard: E.coli; Immunogen sequence: R32-Q220
Target
This gene is mapped to 1q24.2. The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. The protein encoded by this gene antagonizes transcriptional activation and cellular transformation by E1A. This protein shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. This gene may contribute to the transcriptional control of cell growth and differentiation.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human CREG1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CREG1 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: R32-Q220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CREG1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
312 pg/ml - 20,000 pg/ml
Sensitivity
<50 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human CREG1
NCBI Gene Symbol
CREG1
Protein Name
Protein CREG1
Gene Name URL
CREG1
Nucleotide Accession Number
NM_003851.2

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