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Pla2g2a ELISA Kit (Mouse)

CAT: 0247-OKBB01323-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01323-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 56 234 938 58 242 951 Standard deviation 2.69 13.34 43.15 2.96 14.28 60.86 CV (%) 4.8 5.7 4.6 5.1 5.9 6.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 56 234 938 58 242 951 Mean (pg/ml) 56 234 938 58 242 951 Standard deviation 2.69 13.34 43.15 2.96 14.28 60.86 Standard deviation 2.69 13.34 43.15 2.96 14.28 60.86 CV (%) 4.8 5.7 4.6 5.1 5.9 6.4 CV (%) 4.8 5.7 4.6 5.1 5.9 6.4
CAS Number
7732-18-5
Gene Name
Phospholipase A2, group IIA (platelets, synovial fluid)
Gene Aliases
Phospholipase A2, membrane associated; Enhancing factor; EF; GIIC sPLA2; Group IIA phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2A; Pla2g2a
Gene ID
18780
Accession Number
NP_001076000.1
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: N22-C146
Target
Phospholipase A2, membrane associated is an enzyme that in humans is encoded by the PLA2G2A gene. It is mapped to 4 D3; 4 70.57 cM. Proteins belonging to the phospholipase A2 (PLA2) family hydrolyze phospholipids into sn2 fatty acids and lysophospholipids. They function in a variety of cellular processes, including the digestion of phospholipids and the production of molecules that induce inflammatory responses. This gene encodes a member of the group II class of secretory PLA2s. The secreted enzyme binds to heparin on the cell surface. Mutations in this gene increase the occurrence of intestinal polyps caused by a dominant mutation in the adenomatosis polyposis coli gene. A frameshift inactivates this gene product in some mouse strains including the strain of the reference genome, C57BL/6J, whereas a functional protein is produced in other strains.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse PLA2G2A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from sheep specific for PLA2G2A has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: N22-C146) and test samples are added to the wells, a biotinylated detection polyclonal antibody from sheep specific for PLA2G2A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse PLA2G2A
NCBI Gene Symbol
PLA2G2A
Protein Name
Phospholipase A2, membrane associated
Gene Name URL
PLA2G2A
Nucleotide Accession Number
NM_001082531.1

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