BMP10 ELISA Kit (Human)

CAT: 0247-OKBB01235-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
CAT#:0247-OKBB01235-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 207 429 52 231 462 Standard deviation 2.08 11.8 19.8 15.71 16.63 35.57 CV (%) 5.2 5.7 4.6 6.8 7.2 7.7 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 40 207 429 52 231 462 Mean (pg/ml) 40 207 429 52 231 462 Standard deviation 2.08 11.8 19.8 15.71 16.63 35.57 Standard deviation 2.08 11.8 19.8 15.71 16.63 35.57 CV (%) 5.2 5.7 4.6 6.8 7.2 7.7 CV (%) 5.2 5.7 4.6 6.8 7.2 7.7
CAS Number
7732-18-5
Gene Name
Bone morphogenetic protein 10
Gene Aliases
Bone morphogenetic protein 10; BMP-10; BMP10
Gene ID
27302
Accession Number
NP_055297.1
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: N314-R421
Target
Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-β superfamily of structurally related signaling proteins. This gene is mapped to 2p13.3. Members of this superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life.  BMP-10 plays a crucial role in the development of the embryonic heart by acting to stimulate and maintain cardiomyocyte proliferation.  It can signal through various receptor complexes usually containing BMPR-1A, BMPR-1B, ALK1, ALK3, or ALK6. The interaction of BMP-10 with its specific receptors can induce signaling initiated by the phosphorylation of SMAD transcription factors, including SMAD1, SMAD5, or SMAD8, but can also induce SMAD independent processes.  BMP-10 is structurally related to BMP-9, and both can inhibit endothelial cell proliferation and migration.  Recombinant human BMP-10 is a 24.4 kDa homodimeric disulfide-linked protein consisting of two 108 amino acid subunits, which correspond to amino acid residues 317 to 424 of the full-length BMP-10 precursor.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human BMP-10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-10 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: N314-R421) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human BMP-10
NCBI Gene Symbol
BMP10
Protein Name
Bone morphogenetic protein 10
Gene Name URL
BMP10
Nucleotide Accession Number
NM_014482.2

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