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Stat3 ELISA Kit (Mouse)

CAT: 0247-OKBB01197-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01197-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2876 13987 37231 2911 14122 4269 Standard deviation 158.18 713.3 1600.9 177.57 932.05 320.18 CV (%) 5.5 5.1 4.3 6.1 6.6 7.5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2876 13987 37231 2911 14122 4269 Mean (pg/ml) 2876 13987 37231 2911 14122 4269 Standard deviation 158.18 713.3 1600.9 177.57 932.05 320.18 Standard deviation 158.18 713.3 1600.9 177.57 932.05 320.18 CV (%) 5.5 5.1 4.3 6.1 6.6 7.5 CV (%) 5.5 5.1 4.3 6.1 6.6 7.5
CAS Number
7732-18-5
Gene Name
Signal transducer and activator of transcription 3
Gene Aliases
Fibroblast growth factor-binding protein 1; FGF-BP; FGF-BP1; FGF-binding protein 1; FGFBP-1; 17 kDa heparin-binding growth factor-binding protein; 17 kDa HBGF-binding protein; HBp17; FGFBP1; FGFBP; HBP17
Gene ID
20848
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: E25-K413
Target
SERPINA1 is also known as PI, A1A or AAT. This gene is mapped to 14q32.1. The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse AAT ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for AAT has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: E25-K413） and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for AAT is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse AAT amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
1.56 ng/ml - 100 ng/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 15 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse AAT
NCBI Gene Symbol
STAT3
Protein Name
Alpha-1-antitrypsin 1-2
Gene Name URL
STAT3

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