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Hrg ELISA Kit (Mouse)

CAT: 0247-OKBB01196-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01196-96WSize:96 Well With Removable Strips
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1100 4259 314 1299 4301 Standard deviation 12.79 49.5 264.06 17.27 75.34 326.88 CV (%) 4.1 4.5 6.2 5.5 5.8 7.6 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 312 1100 4259 314 1299 4301 Mean (pg/ml) 312 1100 4259 314 1299 4301 Standard deviation 12.79 49.5 264.06 17.27 75.34 326.88 Standard deviation 12.79 49.5 264.06 17.27 75.34 326.88 CV (%) 4.1 4.5 6.2 5.5 5.8 7.6 CV (%) 4.1 4.5 6.2 5.5 5.8 7.6
CAS Number
7732-18-5
Gene Name
Histidine-rich glycoprotein
Gene Aliases
Histidine-rich glycoprotein; Histidine-proline-rich glycoprotein; HPRG; Hrg
Gene ID
94175
Accession Number
NP_444406.2
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: L19-K525
Target
HRG is a glycoprotein that in humans is encoded by the HRG gene. The HRG protein is produced in the liver, and it could also be synthesized by monocytes,  macrophages, and megakaryocytes. It possesses a multi-domain structure, which makes it capable of binding to numerous ligands and modulating various biological processes including immunity,  vascularization and coagulation.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse HRG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for HRG has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: L19-K525） and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HRG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse HRG amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 15 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse HRG
NCBI Gene Symbol
HRG
Protein Name
Histidine-rich glycoprotein
Gene Name URL
HRG
Nucleotide Accession Number
NM_053176.2

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