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UMOD ELISA Kit (Human)

CAT: 0247-OKBB01182-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01182-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 215 1090 5159 226 982 5437 Standard deviation 13.33 57.77 211.51 16.72 62.84 293.59 CV (%) 6.2 5.3 4.1 7.4 6.4 5.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 215 1090 5159 226 982 5437 Mean (pg/ml) 215 1090 5159 226 982 5437 Standard deviation 13.33 57.77 211.51 16.72 62.84 293.59 Standard deviation 13.33 57.77 211.51 16.72 62.84 293.59 CV (%) 6.2 5.3 4.1 7.4 6.4 5.4 CV (%) 6.2 5.3 4.1 7.4 6.4 5.4
CAS Number
7732-18-5
Gene Name
Uromodulin
Gene Aliases
Uromodulin; Tamm-Horsfall urinary glycoprotein; THP; Uromodulin, secreted form; UMOD
Gene ID
7369
Accession Number
NP_001008390.1
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: D25-F587
Target
The Tamm–Horsfall glycoprotein (THP), also known as uromodulin, is a glycoprotein that in humans is encoded by the UMOD gene. The protein encoded by this gene is the most abundant protein in mammalian urine under physiological conditions. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. This protein may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of this protein in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the renal disorders medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN) . Alternative splicing of this gene results in multiple transcript variants.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human Uromodulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Uromodulin has been precoated onto 96-well plates. Standards (NS0, D25-F587) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Uromodulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Uromodulin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human Uromodulin
NCBI Gene Symbol
UMOD
Protein Name
Uromodulin
Gene Name URL
UMOD
Nucleotide Accession Number
NM_001008389.2

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