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Hp ELISA Kit (Mouse)

CAT: 0247-OKBB01181-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01181-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2678 12849 42168 2657 13883 41875 Standard deviation 200.85 976.52 1981.89 207.24 1082.87 2093.75 CV (%) 7.5 7.6 4.7 7.8 7.8 5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2678 12849 42168 2657 13883 41875 Mean (pg/ml) 2678 12849 42168 2657 13883 41875 Standard deviation 200.85 976.52 1981.89 207.24 1082.87 2093.75 Standard deviation 200.85 976.52 1981.89 207.24 1082.87 2093.75 CV (%) 7.5 7.6 4.7 7.8 7.8 5 CV (%) 7.5 7.6 4.7 7.8 7.8 5
CAS Number
7732-18-5
Gene Name
Haptoglobin
Gene Aliases
Haptoglobin; Zonulin; Haptoglobin alpha chain; Haptoglobin beta chain; Hp
Gene ID
15439
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: V19-N347
Target
Haptoglobin (HP), is a protein that in humans is encoded by the HP gene. Haptoglobin, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha and 2 beta polypeptides that are covalently associated by disulfide bonds. Haptoglobin is homologous to serine proteases of the chymotrypsinogen family. A major function of haptoglobin is to bind hemoglobin (Hb) to form a stable Hp-Hb complex and thereby prevent Hb-induced oxidative tissue damage. Haptoglobin is an unusual secretory protein in that it is proteolytically processed in the endoplasmic reticulum and not in the Golgi. In clinical settings, the haptoglobulin assay is used to screen for and monitor intravascular hemolytic anemia .
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse Haptoglobin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Haptoglobin has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: V19-N347) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Haptoglobin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Haptoglobin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
1.56 ng/ml - 100 ng/ml
Sensitivity
<20 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse Haptoglobin
NCBI Gene Symbol
HP
Protein Name
Haptoglobin
Gene Name URL
HP

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