Products for Research Use Only
RegisterLogin

Afp ELISA Kit (Mouse)

CAT: 0247-OKBB01164-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKBB01164-96WSize:96 Well With Removable Strips
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 50 189 834 57 204 928 Standard deviation 2.40 9.26 41.70 2.91 12.04 59.39 CV (%) 4.8 4.9 5.0 5.1 5.9 6.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 50 189 834 57 204 928 Mean (pg/ml) 50 189 834 57 204 928 Standard deviation 2.40 9.26 41.70 2.91 12.04 59.39 Standard deviation 2.40 9.26 41.70 2.91 12.04 59.39 CV (%) 4.8 4.9 5.0 5.1 5.9 6.4 CV (%) 4.8 4.9 5.0 5.1 5.9 6.4
CAS Number
7732-18-5
Gene Name
Alpha fetoprotein
Gene Aliases
Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; Afp
Gene ID
11576
Accession Number
NP_031449.3
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: K19-V605
Target
Alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. This gene is mapped to 5 E1; 5 44.77 cM. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse AFP ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for AFP has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: K19-V605) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for AFP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse AFP amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
312 pg/ml - 20,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse AFP
NCBI Gene Symbol
AFP
Protein Name
Alpha-fetoprotein
Gene Name URL
AFP
Nucleotide Accession Number
NM_007423.4

Related Products

CatalogName
OKCD04403-96WAFP ELISA Kit (Horse)
OKCD06082-96WAFP ELISA Kit (Pig)
OKEH07334-96WAFP ELISA Kit (Chicken)
OKCD00788-96WAFP ELISA Kit (Dog)
OKEH00252-96WAFP ELISA Kit (Rat)
OKEH07333-96WAFP ELISA Kit (Bovine)
OKEH03768-96WAFP ELISA Kit (Pig)
OKCD00787-96WAFP ELISA Kit (Bovine)
OKCD06083-96WAFP ELISA Kit (Rat)
OKEH07335-96WAFP ELISA Kit (Dog)

Alternative Products

CatalogName
GTR10374983Mouse Cholesterol ELISA ELISA
EA-2402Mouse FGFb ELISA
EA-2403Mouse EGF ELISA
EA-2203Mouse TNF-a ELISA
EA-2202Mouse Leptin ELISA
TBS3099Mouse Glucagon ELISA
E109969Mouse Aβ ELISA Kit
E062537Mouse ON ELISA Kit
1037Mouse Nephrin ELISA
YP-W22083-01Mouse AT (AT) ELISA Kit

Popular Products