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AOC3 ELISA Kit (Human)

CAT: 0247-OKBB01153-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01153-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1035 5427 21122 1103 5962 23124 Standard deviation 72.45 260.49 1203.95 80.51 387.53 1364.31 CV (%) 7 4.8 5.7 7.3 6.5 5.9 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1035 5427 21122 1103 5962 23124 Mean (pg/ml) 1035 5427 21122 1103 5962 23124 Standard deviation 72.45 260.49 1203.95 80.51 387.53 1364.31 Standard deviation 72.45 260.49 1203.95 80.51 387.53 1364.31 CV (%) 7 4.8 5.7 7.3 6.5 5.9 CV (%) 7 4.8 5.7 7.3 6.5 5.9
CAS Number
7732-18-5
Gene Name
Amine oxidase, copper containing 3
Gene Aliases
Membrane primary amine oxidase
Gene ID
8639
Accession Number
NP_001264660.1
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: N2-N763
Target
Amine oxidase, copper containing 3, also known as vascular adhesion protein (VAP-1) and HPAO is an enzyme that in humans is encoded by the AOC3 gene on chromosome 17. This protein is a member of the semicarbazide-sensitive amine oxidase (SSAO) family and is associated with many vascular diseases. This gene encodes a member of the semicarbazide-sensitive amine oxidase family. Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The encoded protein is localized to the cell surface, has adhesive properties as well as monoamine oxidase activity, and may be involved in leukocyte trafficking. Alterations in levels of the encoded protein may be associated with many diseases, including diabetes mellitus. A pseudogene of this gene has been described and is located approximately 9-kb downstream on the same chromosome. Alternative splicing results in multiple transcript variants.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human VAP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VAP-1 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: N2-N763) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VAP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human VAP-1 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
0.78 ng/ml - 50 ng/ml
Sensitivity
<20 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human VAP-1
NCBI Gene Symbol
AOC3
Protein Name
Membrane primary amine oxidase
Gene Name URL
AOC3
Nucleotide Accession Number
NM_001277731.1

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