Tff3 ELISA Kit (Mouse)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 77 289 895 74 263 976 Standard deviation 4.62 20.23 59.96 5.25 19.72 68.32 CV (%) 6 7 6.7 7.1 7.5 7 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 77 289 895 74 263 976 Mean (pg/ml) 77 289 895 74 263 976 Standard deviation 4.62 20.23 59.96 5.25 19.72 68.32 Standard deviation 4.62 20.23 59.96 5.25 19.72 68.32 CV (%) 6 7 6.7 7.1 7.5 7 CV (%) 6 7 6.7 7.1 7.5 7

• Gene Name:

  Trefoil factor 3, intestinal

• Gene Aliases:

  Trefoil factor 3; Intestinal trefoil factor; mITF; Tff3; Itf

• Gene ID:

  21786

• Accession Number:

  NP_035705.1

• Reactivity:

  Mouse

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: A23-F81

• Target:

  Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's mouse TFF3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TFF3 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: A23-F81) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFF3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse TFF3 amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernates, serum and plasma (heparin, EDTA)

• Detection Range:

  31.2 pg/ml - 2,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant mouse TFF3

• NCBI Gene Symbol:

  TFF3

• Protein Name:

  Trefoil factor 3

• Gene Name URL:

  TFF3

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_011575.2