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Fabp2 ELISA Kit (Mouse)

CAT: 0247-OKBB01136-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01136-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 75 265 861 69 288 910 Standard deviation 5.92 13.78 38.74 5.93 17.56 56.42 CV (%) 7.9 5.2 4.5 8.6 6.1 6.2 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 75 265 861 69 288 910 Mean (pg/ml) 75 265 861 69 288 910 Standard deviation 5.92 13.78 38.74 5.93 17.56 56.42 Standard deviation 5.92 13.78 38.74 5.93 17.56 56.42 CV (%) 7.9 5.2 4.5 8.6 6.1 6.2 CV (%) 7.9 5.2 4.5 8.6 6.1 6.2
CAS Number
7732-18-5
Gene Name
Fatty acid binding protein 2, intestinal
Gene Aliases
Fatty acid-binding protein, intestinal; Fatty acid-binding protein 2; Intestinal-type fatty acid-binding protein; I-FABP; Fabp2; Fabpi
Gene ID
14079
Accession Number
NP_032006.1
Reactivity
Mouse
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A2-E132
Target
Fatty acid-binding protein 2 (FABP2), also known as Intestinal-type fatty acid-binding protein (I-FABP) is a protein that in humans is encoded by the FABP2 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. Using a human cDNA probe for in situ hybridization studies, they regionalized the assignment to 4q28-q31. The intestinal form of FABP was mapped to mouse chromosome 3 between the amylase and alcohol dehydrogenase-3 loci. Also, they may be responsible in the modulation of cell growth and proliferation.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse FABP2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FABP2 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: A2-E132) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FABP2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse FABP2 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse FABP2
NCBI Gene Symbol
FABP2
Protein Name
Fatty acid-binding protein, intestinal
Gene Name URL
FABP2
Nucleotide Accession Number
NM_007980.3