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Efemp1 ELISA Kit (Mouse)

CAT: 0247-OKBB01113-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01113-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3087 14945 46910 3111 1500 4713 Standard deviation 145.09 821.98 2767.69 199.1 100.5 353.48 CV (%) 4.7 5.5 5.9 6.4 6.7 7.5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 3087 14945 46910 3111 1500 4713 Mean (pg/ml) 3087 14945 46910 3111 1500 4713 Standard deviation 145.09 821.98 2767.69 199.1 100.5 353.48 Standard deviation 145.09 821.98 2767.69 199.1 100.5 353.48 CV (%) 4.7 5.5 5.9 6.4 6.7 7.5 CV (%) 4.7 5.5 5.9 6.4 6.7 7.5
CAS Number
7732-18-5
Gene Name
Epidermal growth factor-containing fibulin-like extracellular matrix protein 1
Gene Aliases
EGF-containing fibulin-like extracellular matrix protein 1; Fibulin-3; FIBL-3; Efemp1; Fbln3
Gene ID
216616
Accession Number
NP_666127.2
Reactivity
Mouse
Immunogen
Expression system for standard: CHO; Immunogen sequence: Q18-F493
Target
EGF-containing fibulin-like extracellular matrix protein 1 is a protein that in humans is encoded by the EFEMP1 gene. This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse EFEMP1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EFEMP1 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO, Immunogen sequence: Q18-F493） and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EFEMP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse EFEMP1 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
312 pg/ml - 20,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 15 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse EFEMP1
NCBI Gene Symbol
EFEMP1
Protein Name
EGF-containing fibulin-like extracellular matrix protein 1
Gene Name URL
EFEMP1
Nucleotide Accession Number
NM_146015.2

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