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Klk1 ELISA Kit (Mouse)

CAT: 0247-OKBB01109-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01109-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 150 602 1476 154 580 1608 Standard deviation 9.15 43.94 116.6 11.08 51.62 136.68 CV (%) 6.1 7.3 7.9 7.2 8.9 8.5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 150 602 1476 154 580 1608 Mean (pg/ml) 150 602 1476 154 580 1608 Standard deviation 9.15 43.94 116.6 11.08 51.62 136.68 Standard deviation 9.15 43.94 116.6 11.08 51.62 136.68 CV (%) 6.1 7.3 7.9 7.2 8.9 8.5 CV (%) 6.1 7.3 7.9 7.2 8.9 8.5
CAS Number
7732-18-5
Gene Name
Kallikrein 1
Gene Aliases
Kallikrein 1, Kallikrein1, Kallikrein-1, klk 1, Klk 6, KLK1, Klk-1, Klk1, P06870, Pancreatic kallikrein
Gene ID
16612
Accession Number
NP_001307260.1
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: I25-D261
Target
Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse KLK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for KLK1 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: I25-D261) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse KLK1 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<12 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse KLK1
NCBI Gene Symbol
KLK1
Protein Name
Kallikrein-1
Gene Name URL
KLK1
Nucleotide Accession Number
NM_001320331.1

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