CHL1 ELISA Kit (Human)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 291 1965 5542 267 2117 5729 Standard deviation 22.11 141.48 343.6 21.09 192.64 435.4 CV (%) 7.6 7.2 6.2 7.9 9.1 7.6 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 291 1965 5542 267 2117 5729 Mean (pg/ml) 291 1965 5542 267 2117 5729 Standard deviation 22.11 141.48 343.6 21.09 192.64 435.4 Standard deviation 22.11 141.48 343.6 21.09 192.64 435.4 CV (%) 7.6 7.2 6.2 7.9 9.1 7.6 CV (%) 7.6 7.2 6.2 7.9 9.1 7.6

• Gene Name:

  Cell adhesion molecule L1 like

• Gene Aliases:

  CALL, Cell adhesion molecule with homology to L1CAM (close homolog of L1), Close homolog of L1, FLJ44930, L1 cell adhesion molecule 2, L1CAM2, MGC132578, Neural cell adhesion molecule, O00533

• Gene ID:

  10752

• Accession Number:

  NP_001240316.1

• Reactivity:

  Human

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: I25-Q1096

• Target:

  Neural cell adhesion molecule L1-like protein also known as close homolog of L1 (CHL1) is a protein that in humans is encoded by the CHL1 gene. This protein is a member of the L1 gene family of neural cell adhesion molecules. By FISH, the CHL1 gene was mapped to chromosome 3p26.1. The protein however has dynamic localisation, meaning that it has not only multiple roles in the cell, but also various locations. This protein may also play a role in the growth of certain cancers.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's human CHL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CHL1 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: I25-Q1096) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CHL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CHL1 amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernates , cell lysates, serum and plasma (heparin, EDTA)

• Detection Range:

  156 pg/ml - 10,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant human CHL1

• NCBI Gene Symbol:

  CHL1

• Protein Name:

  Neural cell adhesion molecule L1-like protein

• Gene Name URL:

  CHL1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001253387.1