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ANGPT2 ELISA Kit (Rat)

CAT: 0247-OKBB01099-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01099-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 253 1986 5096 239 1814 4721 Standard deviation 19.98 99.3 229.32 22.22 121.53 231.32 CV (%) 7.9 5 4.5 9.3 6.7 4.9 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 253 1986 5096 239 1814 4721 Mean (pg/ml) 253 1986 5096 239 1814 4721 Standard deviation 19.98 99.3 229.32 22.22 121.53 231.32 Standard deviation 19.98 99.3 229.32 22.22 121.53 231.32 CV (%) 7.9 5 4.5 9.3 6.7 4.9 CV (%) 7.9 5 4.5 9.3 6.7 4.9
CAS Number
7732-18-5
Gene Name
Angiopoietin 2
Gene Aliases
ANG 2, ANG2, ANG-2, Angiopoietin 2, Angiopoietin2, Angiopoietin-2, Angiopoietin2 (ANG2), Angiopoietin-2 (ANG-2), ANGP2, ANGPT2, O15123
Gene ID
89805
Accession Number
NP_604449.1
Reactivity
Rat
Immunogen
Expression system for standard: NS0; Immunogen sequence: Y19-F496
Target
Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's rat Angiopoietin-2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Angiopoietin-2 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: Y19-F496) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiopoietin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat Angiopoietin-2 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<15 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant rat Angiopoietin-2
NCBI Gene Symbol
ANGPT2
Protein Name
Angiopoietin-2
Gene Name URL
ANGPT2
Nucleotide Accession Number
NM_134454.1

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