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Bmp7 ELISA Kit (Hamster)

CAT: 0247-OKBB00994-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00994-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 185 665 1408 210 887 1699 Standard deviation 9.25 43.89 66.18 14.91 70.96 132.52 CV (%) 5.0 6.6 4.7 7.1 8.0 7.8 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 185 665 1408 210 887 1699 Mean (pg/ml) 185 665 1408 210 887 1699 Standard deviation 9.25 43.89 66.18 14.91 70.96 132.52 Standard deviation 9.25 43.89 66.18 14.91 70.96 132.52 CV (%) 5.0 6.6 4.7 7.1 8.0 7.8 CV (%) 5.0 6.6 4.7 7.1 8.0 7.8
CAS Number
7732-18-5
Gene Name
Bone morphogenetic protein 7
Gene Aliases
Bone morphogenetic protein 7; BMP-7; Osteogenic protein 1; OP-1; Bmp7; Bmp-7, Op1
Gene ID
100759897
Accession Number
NP_031583.2
Reactivity
Hamster
Immunogen
Expression system for standard: CHO; Immunogen sequence: S292-H430
Target
Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-β superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's Hamster BMP-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BMP-7 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO, Immunogen sequence: S292-H430) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Hamster BMP-7 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Chinese hamster BMP-7
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
NCBI Gene Symbol
BMP7
Protein Name
Bone morphogenetic protein 7
Gene Name URL
BMP7
Nucleotide Accession Number
NM_007557.3

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