CD44 ELISA Kit (Mouse)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 723 1936 8300 711 1975 7916 Standard deviation 48.44 143.26 564.4 61.14 179.72 585.78 CV (%) 6.7 7.4 6.8 8.6 9.1 7.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 723 1936 8300 711 1975 7916 Mean (pg/ml) 723 1936 8300 711 1975 7916 Standard deviation 48.44 143.26 564.4 61.14 179.72 585.78 Standard deviation 48.44 143.26 564.4 61.14 179.72 585.78 CV (%) 6.7 7.4 6.8 8.6 9.1 7.4 CV (%) 6.7 7.4 6.8 8.6 9.1 7.4

• Gene Name:

  CD44 molecule (Indian blood group)

• Gene Aliases:

  LHR, BA-1, CD 44, CD44, CD44 antigen, CD44 molecule (Indian blood group), CD44 molecule, CD44_HUMAN, CDw44, Cell surface glycoprotein CD44, chondroitin sulfate proteoglycan 8, CSPG8, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HCELL, hematopoietic cell E- and L-selectin ligand, Heparan sulfate proteoglycan, Hermes antigen, homing function and Indian blood group system, HSA, HUTCH-I, HUTCH1, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH1, PGP-1, PGP-I, PGP1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

• Gene ID:

  12505

• Accession Number:

  NP_001034240.1

• Reactivity:

  Mouse

• Immunogen:

  Expression system for standard: CHO; Immunogen sequence: Q25-T224

• Target:

  CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. It is contains 19 exons spanning 50 kb of genomic DNA. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs) . Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's mouse CD44 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from sheep specific for CD44 has been precoated onto 96-well plates. Standards (CHO, Q25 - T224) and test samples are added to the wells, a biotinylated detection polyclonal antibody from sheep specific for CD44 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CD44 amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)

• Detection Range:

  312 pg/ml - 20,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant mouse CD44

• NCBI Gene Symbol:

  CD44

• Protein Name:

  CD44 antigen

• Gene Name URL:

  CD44

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001039151.1