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FGF23 ELISA Kit (Mouse)

CAT: 0247-OKBB00944-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00944-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 216 449 33 213 449 Standard deviation 2.73 8.85 35.47 2.77 12.78 35.47 CV (%) 7.8 4.1 7.9 8.4 6 7.9 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 216 449 33 213 449 Mean (pg/ml) 35 216 449 33 213 449 Standard deviation 2.73 8.85 35.47 2.77 12.78 35.47 Standard deviation 2.73 8.85 35.47 2.77 12.78 35.47 CV (%) 7.8 4.1 7.9 8.4 6 7.9 CV (%) 7.8 4.1 7.9 8.4 6 7.9
CAS Number
7732-18-5
Gene Name
Fibroblast growth factor 23
Gene Aliases
ADHR, FGF-23, Fgf23, FGF23_HUMAN, FGFN, Fibroblast growth factor 23, Fibroblast growth factor 23 C-terminal peptide, Fibroblast growth factor 23 precursor, HPDR2, HYPF, Phosphatonin, PHPTC, Tumor derived hypophosphatemia inducing factor, Tumor-derived hypophosphatemia-inducing factor
Gene ID
64654
Accession Number
NP_073148.1
Reactivity
Mouse
Immunogen
Expression system for standard: E.coli; Immunogen sequence: Y25-V251
Target
Fibroblast growth factor 23 or FGF23 is a protein that in humans is encoded by the FGF23 gene. It is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 is secreted by Osteocytes in response to elevated Calcitriol. And it acts on the kidneys, where it decreases the expression of NPT2, a sodium-phosphate cotransporter in the proximal tubule. Thus, FGF23 decreases the reabsorption and increases excretion of phosphate. Also, FGF23 may suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse FGF23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for FGF23 has been precoated onto 96-well plates. Standards (E.coli, Y25-V251) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse FGF23 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse FGF23
NCBI Gene Symbol
FGF23
Protein Name
Fibroblast growth factor 23
Gene Name URL
FGF23
Nucleotide Accession Number
NM_022657.4

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