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S100A12 ELISA Kit (Human)

CAT: 0247-OKBB00910-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00910-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 58 265 947 56 247 855 Standard deviation 2.32 20.4 67.23 3.08 23.46 68.4 CV (%) 4 7.7 7.1 5.5 9.5 8 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 58 265 947 56 247 855 Mean (pg/ml) 58 265 947 56 247 855 Standard deviation 2.32 20.4 67.23 3.08 23.46 68.4 Standard deviation 2.32 20.4 67.23 3.08 23.46 68.4 CV (%) 4 7.7 7.1 5.5 9.5 8 CV (%) 4 7.7 7.1 5.5 9.5 8
CAS Number
7732-18-5
Gene Name
S100 calcium binding protein A12
Gene Aliases
CAAF1, CAGC, Calcitermin, Calcium binding protein in amniotic fluid, Calcium-binding protein in amniotic fluid 1, Calgranulin C, Calgranulin related protein, Calgranulin-C, Calgranulin-related protein, CGRP, EN RAGE, EN-RAGE, ENRAGE, Extracellular newly identified RAGE-binding protein, migration inhibitory factor-related protein 6, MRP6, Neutrophil S100 protein, p6, Protein S100 A12, S100 calcium binding protein A12, S100 calcium-binding protein A12 (calgranulin C), S100 calcium-binding protein A12, S100A12, S10AC_HUMAN
Gene ID
6283
Accession Number
NP_005612.1
Reactivity
Human
Immunogen
Expression system for standard: E.coli; Immunogen sequence: M1-E92
Target
S100 calcium-binding protein A12 (S100A12) is a protein that in humans is encoded by the S100A12 gene. It is also known as calgranulin C. By direct R-banding FISH, the S100A12 gene is mapped to chromosome 1q21.2-q22. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. And this protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human S100A12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for S100A12 has been precoated onto 96-well plates. Standards (E.coli, M1-E92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for S100A12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human S100A12 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human S100A12
NCBI Gene Symbol
S100A12
Protein Name
Protein S100-A12
Gene Name URL
S100A12
Nucleotide Accession Number
NM_005621.1

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