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S100A8 ELISA Kit (Mouse)

CAT: 0247-OKBB00904-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00904-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 276 1214 4039 294 1228 4594 Standard deviation 11.59 52.20 165.60 19.40 83.50 326.17 CV (%) 4.2 4.3 4.1 6.6 6.8 7.1 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 276 1214 4039 294 1228 4594 Mean (pg/ml) 276 1214 4039 294 1228 4594 Standard deviation 11.59 52.20 165.60 19.40 83.50 326.17 Standard deviation 11.59 52.20 165.60 19.40 83.50 326.17 CV (%) 4.2 4.3 4.1 6.6 6.8 7.1 CV (%) 4.2 4.3 4.1 6.6 6.8 7.1
CAS Number
7732-18-5
Gene Name
S100 calcium binding protein A8
Gene Aliases
CAGA, CAGA, CFAG, MRP8, Caga, Mrp8, Calgranulin-A, Calprotectin L1L subunit, CFAG, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP 8, MRP-8, P05109, Protein S100A8, Protein S100-A8, S100 calcium binding protein A8, S100 calcium-binding protein A8, S10A8, Urinary stone protein band A
Gene ID
20201
Accession Number
NP_038678.1
Reactivity
Mouse
Immunogen
Expression system for standard: E.coli; Immunogen sequence: P2-E89
Target
S100 calcium-binding protein A8 (S100A8), also known as calgranulin A, is a protein that in humans is encoded by the S100A8 gene. It is mapped to chromosome 1q21.3 based on an alignment of the S100A8 sequence with the genomic sequence. The proteins S100A8 and S100A9 form a heterodimer called calprotectin which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. This gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. And S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse S100A8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for S100A8 has been precoated onto 96-well plates. Standards (E.coli, P2-E89) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for S100A8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse S100A8 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<50 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse S100A8
NCBI Gene Symbol
S100A8
Protein Name
Protein S100-A8
Gene Name URL
S100A8
Nucleotide Accession Number
NM_013650.2

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