IGFBP7 ELISA Kit (Monkey)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (ng/ml) 2.3 16.2 28.1 2.8 15.4 29.7 Standard deviation 0.101 0.729 1.43 0.188 1.063 2.14 CV (%) 4.4 4.5 7.2 6.7 6.9 7.2 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (ng/ml) 2.3 16.2 28.1 2.8 15.4 29.7 Mean (ng/ml) 2.3 16.2 28.1 2.8 15.4 29.7 Standard deviation 0.101 0.729 1.43 0.188 1.063 2.14 Standard deviation 0.101 0.729 1.43 0.188 1.063 2.14 CV (%) 4.4 4.5 7.2 6.7 6.9 7.2 CV (%) 4.4 4.5 7.2 6.7 6.9 7.2

• Gene Name:

  Insulin-like growth factor-binding protein 7

• Gene Aliases:

  Insulin-like growth factor-binding protein 7; IBP-7; IGF-binding protein 7; IGFBP-7; IGFBP-rP1; MAC25 protein; PGI2-stimulating factor; Prostacyclin-stimulating factor; Tumor-derived adhesion factor; TAF; IGFBP7; MAC25, PSF

• Gene ID:

  693564

• Accession Number:

  NP_001240764.1

• Reactivity:

  Monkey

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: D30-L282

• Target:

  Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. The gene was mapped to chromosome 4q12. The major function of the protein is the regulation of availability of insulin-like growth factors (IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's Monkey IGFBP7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP7 has been precoated onto 96-well plates. Standards (NS0, D30-L282) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Monkey IGFBP7 amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Sandwich High Sensitivity ELISA kit for Quantitative Detection of monkey primate IGFBP7

• Detection Range:

  625 pg/ml - 40,000 pg/ml

• Sensitivity:

  <20 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• NCBI Gene Symbol:

  IGFBP7

• Protein Name:

  Insulin-like growth factor-binding protein 7

• Gene Name URL:

  IGFBP7

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001253835.1