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IL22 ELISA Kit (Human)

CAT: 0247-OKBB00856-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00856-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 215 435 41 220 427 Standard deviation 2.04 14.83 26.97 2.58 18.92 27.32 CV (%) 5.1 6.9 6.2 6.3 8.6 6.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 40 215 435 41 220 427 Mean (pg/ml) 40 215 435 41 220 427 Standard deviation 2.04 14.83 26.97 2.58 18.92 27.32 Standard deviation 2.04 14.83 26.97 2.58 18.92 27.32 CV (%) 5.1 6.9 6.2 6.3 8.6 6.4 CV (%) 5.1 6.9 6.2 6.3 8.6 6.4
CAS Number
7732-18-5
Gene Name
Interleukin 22
Gene Aliases
Cytokine Zcyto18, IL 21, IL 22, IL D110, IL-10-related T-cell-derived-inducible factor, IL-22, IL-TIF, Il22, IL22_HUMAN, ILTIF, Interleukin 22, Interleukin-22, TIFa, TIFIL 23, zcyto18
Gene ID
50616
Accession Number
NP_065386.1
Reactivity
Human
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A34-I179
Target
Interleukin-22 (IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human IL-22 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-22 has been precoated onto 96-well plates. Standards (E.coli, A34-I179) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-22 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human IL-22 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human IL-22
NCBI Gene Symbol
IL22
Protein Name
Interleukin-22
Gene Name URL
IL22
Nucleotide Accession Number
NM_020525.4

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