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TNFSF13 ELISA Kit (Monkey)

CAT: 0247-OKBB00848-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00848-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 653 2945 5633 864 3214 5366 Standard deviation 26.8 156.1 219.7 42.4 202.5 268.3 CV (%) 4.1 5.3 3.9 4.9 6.3 5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 653 2945 5633 864 3214 5366 Mean (pg/ml) 653 2945 5633 864 3214 5366 Standard deviation 26.8 156.1 219.7 42.4 202.5 268.3 Standard deviation 26.8 156.1 219.7 42.4 202.5 268.3 CV (%) 4.1 5.3 3.9 4.9 6.3 5 CV (%) 4.1 5.3 3.9 4.9 6.3 5
CAS Number
7732-18-5
Gene Name
Tumor necrosis factor (ligand) superfamily, member 13
Gene Aliases
Tumor necrosis factor (Ligand) superfamily member 13 transcript variant delta ; Tumor necrosis factor ligand superfamily member 13 ; TNFSF13
Gene ID
100551507
Accession Number
NP_001185551.1
Reactivity
Monkey
Immunogen
Expression system for standard: NS0; Immunogen sequence: A105-L250
Target
Tumor necrosis factor ligand superfamily member 13 (TNFSF13) also known as a proliferation-inducing ligand (APRIL) is a protein that in humans is encoded by the TNFSF13 gene. TNFSF13 has also been designated CD256 (cluster of differentiation 256) . The protein encoded by this gene is a member of the tumor necrosis factor ligand (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vivo experiments suggest an important role for APRIL in the long-term survival of plasma cells in the bone marrow. Mice deficient in April showed a reduced ability to support plasma cell survival In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such asTNFRSF6/FAS and TNFRSF14/HVEM. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. The TNFSF13 gene lies 878 bp downstream of the TNFSF12 gene on chromosome 17p13.1.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's Monkey TNFSF13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFSF13 has been precoated onto 96-well plates. Standards (NS0, A105-L250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Monkey TNFSF13 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Sandwich High Sensitivity ELISA kit for Quantitative Detection of monkey primate TNFSF13/APRIL
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
NCBI Gene Symbol
TNFSF13
Protein Name
Tumor necrosis factor ligand superfamily member 13
Gene Name URL
TNFSF13
Nucleotide Accession Number
NM_001198622.1

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