THBS1 ELISA Kit (Monkey)

CAT: 0247-OKBB00840-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
CAT#:0247-OKBB00840-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (ng/ml) 15.5 32 52 16.5 38 64 Standard deviation 0.93 1.76 2.08 1.27 2.47 4.61 CV (%) 6 5.5 4 7.8 6.5 7.2 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (ng/ml) 15.5 32 52 16.5 38 64 Mean (ng/ml) 15.5 32 52 16.5 38 64 Standard deviation 0.93 1.76 2.08 1.27 2.47 4.61 Standard deviation 0.93 1.76 2.08 1.27 2.47 4.61 CV (%) 6 5.5 4 7.8 6.5 7.2 CV (%) 6 5.5 4 7.8 6.5 7.2
CAS Number
7732-18-5
Gene Name
Thrombospondin-1
Gene Aliases
Thrombospondin-1; THBS1; TSP, TSP1
Gene ID
705413
Accession Number
NP_003237.2
Reactivity
Monkey
Immunogen
Expression system for standard: NS0; Immunogen sequence: N19-P1170
Target
Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to monkey primate 15q15 and the cognate gene to monkey primate chromosome 2 (region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's Monkey THBS1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for THBS1 has been precoated onto 96-well plates. Standards (NS0, N19-P1170) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for THBS1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Monkey THBS1 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Sandwich High Sensitivity ELISA kit for Quantitative Detection of monkey primate THBS1/TSP1
Detection Range
1.56 ng/ml - 100 ng/ml
Sensitivity
<50 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
NCBI Gene Symbol
THBS1
Protein Name
Thrombospondin-1
Gene Name URL
THBS1
Nucleotide Accession Number
NM_003246.3

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