TPSAB1 ELISA Kit (Human)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 276 1887 5608 283 2054 5485 Standard deviation 18.49 143.41 420.6 23.48 191.02 488.16 CV (%) 6.7 7.6 7.5 8.3 9.3 8.9 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 276 1887 5608 283 2054 5485 Mean (pg/ml) 276 1887 5608 283 2054 5485 Standard deviation 18.49 143.41 420.6 23.48 191.02 488.16 Standard deviation 18.49 143.41 420.6 23.48 191.02 488.16 CV (%) 6.7 7.6 7.5 8.3 9.3 8.9 CV (%) 6.7 7.6 7.5 8.3 9.3 8.9

7732-18-5

Tryptase alpha/beta 1/beta 2

Tryptase-1, Tryptase1, Tryptase 1, TPS1, TPS2, TPSB1, Tryptase-2, Tryptase2, Tryptase 2, Tryptase-II, TryptaseII, Tryptase II, TPSB2, Tryptase alpha/beta-1, Tryptase beta-2

7177; 64499

Human

Expression system for standard: NS0; Immunogen sequence: M1-V275

Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.

ELISA Kit

ELISA

Aviva's human Tryptase ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for Tryptase has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: M1-V275) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Tryptase is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Tryptase amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)

156 pg/ml - 10,000 pg/ml

<15 pg/ml

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Natural and recombinant human Tryptase

TPSAB1/TPSB2

Tryptase alpha/beta-1/beta-2