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FGF7 ELISA Kit (Monkey)

CAT: 0247-OKBB00825-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
CAT#:0247-OKBB00825-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 156 645 1278 147 649 1226 Standard deviation 6.40 27.09 70.29 12.35 39.59 78.46 CV (%) 4.1 4.2 5.6 8.4 6.1 6.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 156 645 1278 147 649 1226 Mean (pg/ml) 156 645 1278 147 649 1226 Standard deviation 6.40 27.09 70.29 12.35 39.59 78.46 Standard deviation 6.40 27.09 70.29 12.35 39.59 78.46 CV (%) 4.1 4.2 5.6 8.4 6.1 6.4 CV (%) 4.1 4.2 5.6 8.4 6.1 6.4
CAS Number
7732-18-5
Gene Name
Fibroblast growth factor 7
Gene Aliases
Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; FGF7; KGF
Gene ID
574345
Accession Number
NP_002000.1
Reactivity
Monkey
Immunogen
Expression system for standard: E.coli; Immunogen sequence: C32-T194
Target
Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of monkey primate and monkey primate homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's Monkey FGF7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for FGF7 has been precoated onto 96-well plates. Standards (E.coli, C32-T194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Monkey FGF7 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Sandwich High Sensitivity ELISA kit for Quantitative Detection of monkey primate FGF7/KGF
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<4 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
NCBI Gene Symbol
FGF7
Protein Name
Fibroblast growth factor 7
Gene Name URL
FGF7
Nucleotide Accession Number
NM_002009.3

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