Flt1 ELISA Kit (Mouse)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 210 954 6138 191 959 6693 Standard deviation 8.82 66.78 349.86 11.26 74.8 468.51 CV (%) 4.2 7 5.7 5.9 7.8 7 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 210 954 6138 191 959 6693 Mean (pg/ml) 210 954 6138 191 959 6693 Standard deviation 8.82 66.78 349.86 11.26 74.8 468.51 Standard deviation 8.82 66.78 349.86 11.26 74.8 468.51 CV (%) 4.2 7 5.7 5.9 7.8 7 CV (%) 4.2 7 5.7 5.9 7.8 7

• Gene Name:

  FMS-like tyrosine kinase 1

• Gene Aliases:

  FLT 1, FLT1, Flt-1, sflt 1, sFLT1, Soluble VEGFR1 variant 2, Soluble VEGFR1 variant 21, sVEGFR1, Tyrosine protein kinase FRT, Tyrosine-protein kinase FRT, VEGF Receptor 1, VEGFR 1, VEGFR-1, VGFR1

• Gene ID:

  14254

• Accession Number:

  NP_034358.2

• Reactivity:

  Mouse

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: Y23-E759

• Target:

  Vascular endothelial growth factor receptor 1 is a protein that in humans is encoded by the FLT1 gene. Oncogene FLT belongs to the src gene family and is related to oncogene ROS. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's mouse VEGFR1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for VEGFR1 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: Y23-E759) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGFR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse VEGFR1 amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernates and serum

• Detection Range:

  156 pg/ml - 10,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant mouse VEGFR1

• NCBI Gene Symbol:

  FLT1

• Protein Name:

  Vascular endothelial growth factor receptor 1

• Gene Name URL:

  FLT1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_010228.3