LIF ELISA Kit (Mouse)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 70 272 15 76 278 Standard deviation 0.7 3.01 19.58 0.72 3.34 24.74 CV (%) 4.4 4.3 7.2 4.8 4.4 8.9 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 16 70 272 15 76 278 Mean (pg/ml) 16 70 272 15 76 278 Standard deviation 0.7 3.01 19.58 0.72 3.34 24.74 Standard deviation 0.7 3.01 19.58 0.72 3.34 24.74 CV (%) 4.4 4.3 7.2 4.8 4.4 8.9 CV (%) 4.4 4.3 7.2 4.8 4.4 8.9

• Gene Name:

  Leukemia inhibitory factor

• Gene Aliases:

  CDF, Cholinergic differentiation factor, D factor, DIA, Differentiation inhibitory activity, Differentiation stimulating factor, Differentiation-stimulating factor, Emfilermin, Hepatocyte stimulating factor III, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, Melanoma-derived LPL inhibitor, MLPL I, P15018

• Gene ID:

  16878

• Accession Number:

  NP_001034626.1

• Reactivity:

  Mouse

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: S24-F203

• Target:

  Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's mouse LIF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for LIF has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: S24-F203) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LIF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse LIF amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernates, serum and plasma (heparin, EDTA)

• Detection Range:

  7.8 pg/ml - 500 pg/ml

• Sensitivity:

  <10 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant mouse LIF

• NCBI Gene Symbol:

  LIF

• Protein Name:

  Leukemia inhibitory factor

• Gene Name URL:

  LIF

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001039537.2