ACE2 ELISA Kit (Mouse)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 417 1235 2279 503 1327 2305 SD 20.85 65.46 127.62 37.73 102.18 189.01 CV% 5 5.3 5.6 7.5 7.7 8.2 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 417 1235 2279 503 1327 2305 Mean (pg/mL) 417 1235 2279 503 1327 2305 SD 20.85 65.46 127.62 37.73 102.18 189.01 SD 20.85 65.46 127.62 37.73 102.18 189.01 CV% 5 5.3 5.6 7.5 7.7 8.2 CV% 5 5.3 5.6 7.5 7.7 8.2

• Gene Name:

  Angiotensin I converting enzyme 2

• Gene Aliases:

  ACE 2, ACE related carboxypeptidase, ACE-related carboxypeptidase, ACE2, ACE2_HUMAN, ACEH, Angiotensin converting enzyme 2, Angiotensin converting enzyme homolog, Angiotensin converting enzyme like protein, Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2, Angiotensin I converting enzyme 2, Angiotensin-converting enzyme homolog, DKFZP434A014, EC 3.4.17, metalloprotease MPROT 15, Metalloprotease MPROT15, OTTHUMP00000022963, Processed angiotensin-converting enzyme 2

• Gene ID:

  70008

• Accession Number:

  NP_001123985.1

• Reactivity:

  Mouse

• Immunogen:

  Expression system for standard: CHO; Immunogen sequence: Q18-T740

• Target:

  AngiotensinI-converting enzyme 2 (ACE 2) is a protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's mouse ACE2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ACE2 has been precoated onto 96-well plates. Standards (CHO, Q18-T740) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ACE2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse ACE2 amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernatants, serum and plasma (heparin, EDTA)

• Detection Range:

  62.5 pg/ml - 4,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant mouse ACE2

• NCBI Gene Symbol:

  ACE2

• Protein Name:

  Angiotensin-converting enzyme 2

• Gene Name URL:

  ACE2

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001130513.1