Products for Research Use Only
RegisterLogin

CXCL3 ELISA Kit (Rat)

CAT: 0247-OKBB00628-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKBB00628-96WSize:96 Well With Removable Strips
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 170 375 602 161 352 593 SD 13.26 19.5 27.09 13.36 22.53 27.87 CV% 7.8 5.2 4.5 8.3 6.4 4.7 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 170 375 602 161 352 593 Mean (pg/mL) 170 375 602 161 352 593 SD 13.26 19.5 27.09 13.36 22.53 27.87 SD 13.26 19.5 27.09 13.36 22.53 27.87 CV% 7.8 5.2 4.5 8.3 6.4 4.7 CV% 7.8 5.2 4.5 8.3 6.4 4.7
CAS Number
7732-18-5
Gene Name
Chemokine (C-X-C motif) ligand 3
Gene Aliases
C-X-C motif chemokine 3, Chemokine (CXC motif) ligand 3, Cinc 2, CINC 2b, Cinc2, CINC2b, CXCL 3, Cxcl3, CXCL3_HUMAN, Cytokine induced neutrophil chemoattractant 2, Dcip1, Dendritic cell inflammatory protein 1, Gm1960, GRO protein gamma, GRO-gamma, GRO-gamma (1-73), GRO-gamma (5-73), GRO3, GRO3 oncogene, GROG, Growth regulated protein gamma, Growth-regulated protein gamma, Macrophage inflammatory protein 2 beta precursor, Macrophage inflammatory protein 2-beta, Melanoma growth stimulatory activity gamma, Member 3, MGSA gamma, MIP 2b, MIP2-beta, MIP2B, SCYB3, Small inducible cytokine subfamily B
Gene ID
171551
Accession Number
NP_612531.1
Reactivity
Rat
Immunogen
Expression system for standard: E.coli; Immunogen sequence: R33-S101
Target
Chemokine (C-X-C motif) ligand 3 (CXCL3) is a small cytokine belonging to the CXC chemokine family. It is mapped to 14p2 in rat. CXCL3 controls migration and adhesion of monocytes and mediates its effects on its target cell by interacting with a cell surface chemokine receptor. It has been shown that CXCL3 regulates cell autonomously the migration of the precursors of cerebellar granule neurons toward the internal layers of cerebellum, during the morphogenesis of cerebellum. CXCL3 also play fundamental roles in the development, homeostasis and it has effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's rat CXCL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL3 has been precoated onto 96-well plates. Standards (E.coli, R33-S101) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat CXCL3 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant rat CXCL3
NCBI Gene Symbol
CXCL3
Protein Name
C-X-C motif chemokine 3
Gene Name URL
CXCL3
Nucleotide Accession Number
NM_138522.1

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number7732-18-5
PubChem CID962
IUPAC Nameoxidane
Molecular FormulaH2O
Molecular Weight18.015
XLogP-0.5
Topological Polar Surface Area1
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Heavy Atom Count1
Formal Charge0
Complexity0
SMILES
O
InChI
InChI=1S/H2O/h1H2
InChIKey
XLYOFNOQVPJJNP-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Water
CAS: 7732-18-5
CID: 962
Formula: H2O
MW: 18.015
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight18.015
XLogP3-0.5
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Exact Mass18.010564683
Monoisotopic Mass18.010564683
Topological Polar Surface Area1 Ų
Heavy Atom Count1
Formal Charge0
Complexity0
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

Related Products

CatalogName
OKBB00945-96WCXCL3 ELISA Kit (Mouse)
OKCD07520-96WCXCL3 ELISA Kit (Human)
OKEH00399-96WCXCL3 ELISA Kit (Human)
OKEH02982-96WCXCL3 ELISA Kit (Mouse)
OKCD07520CXCL3 ELISA Kit (Human) (OKCD07520)
OKEH02982CXCL3 ELISA Kit (Mouse) (OKEH02982)
OKAN05071CXCL3 ELISA Kit (Human) (OKAN05071)
OKBB00945CXCL3 ELISA Kit (Mouse) (OKBB00945)
OKEH00399CXCL3 ELISA Kit (Human) : 96 Wells (OKEH00399)
OACD04010-1MLCXCL3 Antibody

Alternative Products

CatalogName
GTR18799800Rat CXCL3 ELISA Kit
MBS177540-01Rat CXCL3 PicoKine ELISA Kit
EK1373Rat CXCL3 ELISA Kit PicoKine®
E106917Rat GROγ; CXCL3 ELISA Kit
352802CXCL3, BioAssayTM ELISA Kit (Rat)
MBS8421411-01Rat Chemokine Ligand CXCL3 ELISA Kit
EK1373-carrier-freeRat CXCL3 ELISA Kit PicoKine®, Carrier-free
MBS2610723-01Rat CXCL3/CINC-2a/b ELISA Kit
DY516Rat CXCL3/CINC-2 alpha/beta DuoSet ELISA
YP-W36160-01Rat CXC-chemokine ligand 3 (CXCL3) ELISA Kit

Popular Products