Products for Research Use Only
RegisterLogin

ADAMTS4 ELISA Kit (Human)

CAT: 0247-OKBB00627-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKBB00627-96WSize:96 Well With Removable Strips
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 5.9 12 21 6.5 14 23 SD 0.248 0.612 1.2 0.377 0.882 1.66 CV% 4.2 5.1 5.7 5.8 6.3 7.2 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 5.9 12 21 6.5 14 23 Mean (pg/mL) 5.9 12 21 6.5 14 23 SD 0.248 0.612 1.2 0.377 0.882 1.66 SD 0.248 0.612 1.2 0.377 0.882 1.66 CV% 4.2 5.1 5.7 5.8 6.3 7.2 CV% 4.2 5.1 5.7 5.8 6.3 7.2
CAS Number
7732-18-5
Gene Name
ADAM metallopeptidase with thrombospondin type 1 motif, 4
Gene Aliases
A disintegrin and metalloproteinase with thrombospondin motifs 4, A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4, A disintegrin like and metalloprotease with thrombospondin type 1 motif 4, ADAM metallopeptidase with thrombospondin type 1 motif 4, ADAM TS 4, ADAM TS4, ADAM-TS 4, ADAM-TS4, ADAMTS 2, ADAMTS 4, ADAMTS 4, ADAMTS-4, ADAMTS2, ADAMTS4, ADAMTS4 protein, ADMP 1, ADMP-1, ADMP1, Aggrecanase 1, Aggrecanase 1, Aggrecanase-1, Aggrecanase1, ATS4_HUMAN, KIAA0688, KIAA0688
Gene ID
9507
Accession Number
NP_005090.3
Reactivity
Human
Immunogen
Expression system for standard: CHO; Immunogen sequence: F213-C685
Target
ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human ADAMTS4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS4 has been precoated onto 96-well plates. Standards (CHO, F213-C685) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human ADAMTS4 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
625 pg/ml - 40,000 pg/ml
Sensitivity
<20 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human ADAMTS4
NCBI Gene Symbol
ADAMTS4
Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 4
Gene Name URL
ADAMTS4
Nucleotide Accession Number
NM_005099.4

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number7732-18-5
PubChem CID962
IUPAC Nameoxidane
Molecular FormulaH2O
Molecular Weight18.015
XLogP-0.5
Topological Polar Surface Area1
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Heavy Atom Count1
Formal Charge0
Complexity0
SMILES
O
InChI
InChI=1S/H2O/h1H2
InChIKey
XLYOFNOQVPJJNP-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Water
CAS: 7732-18-5
CID: 962
Formula: H2O
MW: 18.015
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight18.015
XLogP3-0.5
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Exact Mass18.010564683
Monoisotopic Mass18.010564683
Topological Polar Surface Area1 Ų
Heavy Atom Count1
Formal Charge0
Complexity0
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

Related Products

CatalogName
OKEH05322-96WADAMTS4 ELISA Kit (Mouse)
OKCD09243-96WADAMTS4 ELISA Kit (Rat)
OKEH08427-96WADAMTS4 ELISA Kit (Bovine)
OKEH03412-96WADAMTS4 ELISA Kit (Rat)
OKEH05322ADAMTS4 ELISA Kit (Mouse) (OKEH05322)
OKEH08427ADAMTS4 ELISA Kit (Bovine) (OKEH08427)
OKCD09244ADAMTS4 ELISA Kit (Rabbit) (OKCD09244)
OKEH03412ADAMTS4 ELISA Kit (Rat) (OKEH03412)
OKCD09243ADAMTS4 ELISA Kit (Rat) (OKCD09243)
OKCD09244-96WOKCD09244-96W - ADAMTS4 ELISA Kit (Rabbit)

Alternative Products

CatalogName
GTR18838738Human ADAMTS4 ELISA Kit
EK1372Human ADAMTS4 ELISA Kit PicoKine®
MBS177535-01Human ADAMTS4 PicoKine ELISA Kit
NBP2-66443Human ADAMTS4 ELISA Kit (Chemiluminescence)
DY4307-05Human ADAMTS4 DuoSet ELISA 5 Plate
GTR18827835-01Human ADAMTS4 ELISA Kit (Ready to Use)
YP-W12482-01Human A Disintegrin And Metalloprotease 4 (ADAMTS4) ELISA Kit
EK1372-carrier-freeHuman ADAMTS4 ELISA Kit PicoKine®, Carrier-free
CRH6314-01ADAMTS4 siRNA (Human)
ANT-35547-100ugHuman ADAMTS4 Antibody

Popular Products