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Thioredoxin ELISA Kit (Human)

CAT: 0247-OKBB00563-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00563-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 0.89 3.62 6.83 0.87 3.85 7.12 SD 0.05 0.17 0.37 0.07 0.24 0.41 CV% 5.6 4.7 5.4 8 6.2 5.8 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 0.89 3.62 6.83 0.87 3.85 7.12 Mean (pg/mL) 0.89 3.62 6.83 0.87 3.85 7.12 SD 0.05 0.17 0.37 0.07 0.24 0.41 SD 0.05 0.17 0.37 0.07 0.24 0.41 CV% 5.6 4.7 5.4 8 6.2 5.8 CV% 5.6 4.7 5.4 8 6.2 5.8
CAS Number
7732-18-5
Gene Name
Thioredoxin
Gene Aliases
ADF, ATL-derived factor, DKFZp686B1993, MGC61975, SASP, Surface associated sulphydryl protein, Surface-associated sulphydryl protein, THIO_HUMAN, Thioredoxin, TRDX, TRX 1, Trx, TRX1, TXN, TXN protein
Gene ID
7295
Accession Number
NP_001231867.1
Reactivity
Human
Immunogen
Expression system for standard: E.coli; Immunogen sequence: V2-V105
Target
Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. They can also act as electron donors to peroxidases and ribonucleotide reductase. Thioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It plays a role in many important biological processes, including redox signaling. This gene also plays a central role in humans and is increasingly linked to medicine through their response to reactive oxygen species (ROS) . VDUP1 is a key stress-responsive inhibitor of Thioredoxin activity in cardiomyocytes.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human Thioredoxin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for Thioredoxin has been precoated onto 96-well plates. Standards (E.coli, V2-V105) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Thioredoxin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Thioredoxin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, serum and plasma (heparin)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human Thioredoxin
NCBI Gene Symbol
TXN
Protein Name
Thioredoxin
Gene Name URL
TXN
Nucleotide Accession Number
NM_001244938.1

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