TLR2 ELISA Kit (Mouse)

CAT: 0247-OKBB00506-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
CAT#:0247-OKBB00506-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 1.05 2.83 5.62 1.38 3.22 5.79 SD 0.036 0.116 0.253 0.077 0.206 0.388 CV% 3.4 4.1 4.5 5.6 6.4 6.7 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 1.05 2.83 5.62 1.38 3.22 5.79 Mean (pg/mL) 1.05 2.83 5.62 1.38 3.22 5.79 SD 0.036 0.116 0.253 0.077 0.206 0.388 SD 0.036 0.116 0.253 0.077 0.206 0.388 CV% 3.4 4.1 4.5 5.6 6.4 6.7 CV% 3.4 4.1 4.5 5.6 6.4 6.7
CAS Number
7732-18-5
Gene Name
Toll-like receptor 2
Gene Aliases
CD_antigen=CD282, CD282, TIL4, Toll like receptor 2, Toll/interleukin 1 receptor like protein 4
Gene ID
24088
Accession Number
NP_036035.3
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q25-L590
Target
Toll-like receptor 2 also known as TLR-2 is a protein that in humans is encoded by the TLR2 gene. TLR2 has also been designated as CD282 (cluster of differentiation 282) . TLR-2 plays a role in the immune system. TLR-2 is a membrane protein, a receptor, which is expressed on the surface of certain cells and recognizes foreign substances and passes on appropriate signals to the cells of the immune system. TLR2 expresses as 4- and 4.4-kb mRNAs in heart, brain, and muscle.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse TLR2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TLR2 has been precoated onto 96-well plates. Standards (NS0, Q25-L590) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TLR2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse TLR2 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse TLR2
NCBI Gene Symbol
TLR2
Protein Name
Toll-like receptor 2
Gene Name URL
TLR2
Nucleotide Accession Number
NM_011905.3

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