TNFRSF13C/BAFFR ELISA Kit (Human)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 643 1604 3262 745 1862 3657 SD 29.6 57.75 169.6 43.21 91.3 234.1 CV% 4.6 3.6 5.2 5.8 4.9 6.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 643 1604 3262 745 1862 3657 Mean (pg/mL) 643 1604 3262 745 1862 3657 SD 29.6 57.75 169.6 43.21 91.3 234.1 SD 29.6 57.75 169.6 43.21 91.3 234.1 CV% 4.6 3.6 5.2 5.8 4.9 6.4 CV% 4.6 3.6 5.2 5.8 4.9 6.4

• Gene Name:

  Tumor necrosis factor receptor superfamily, member 13C

• Gene Aliases:

  B Cell Activating Factor Receptor, B-cell-activating factor receptor, BAFF R, BAFF receptor, BAFF-R, BAFFR, BLyS receptor 3, BlySR3, BR 3, BR3, BROMIX, CD 268, CD268, CD268 antigen, CVID4, MGC138235, OTTHUMP00000028746, Prolixin, TNFRSF 13C, TNFRSF13C, TR13C_HUMAN, Tumor Necrosis Factor Receptor Subunit Member 13C, Tumor necrosis factor receptor superfamily member 13C

• Gene ID:

  115650

• Accession Number:

  NP_443177.1

• Reactivity:

  Human

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: S7-A71

• Target:

  Tumor necrosis factor receptor superfamily member 13C also known as B-cell-activating factor receptor or BAFF receptor is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's human TNFRSF13C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for TNFRSF13C has been precoated onto 96-well plates. Standards (NS0, S7-A71) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF13C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human TNFRSF13C amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

• Detection Range:

  78 pg/ml - 5,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant human TNFRSF13C

• NCBI Gene Symbol:

  TNFRSF13C

• Protein Name:

  Tumor necrosis factor receptor superfamily member 13C

• Gene Name URL:

  TNFRSF13C

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_052945.3